Faculty Research Interests

Affiliates

Lecturers

Faculty Office Hours

Faculty Awards

NMR/Mass Spec Facilities

Reserve NMR

Reserve Mass Spec

X-Ray Facility

X-Ray Crystallography

Resources

Chemistry and Biochemistry
University of California
230 Physical Sciences Bldg.
Santa Cruz, CA 95064
Phone: (831) 459-4002
Fax: (831) 459-2935
Email
Maps & Directions

Thomas Schleich Professor of Chemistry B.S., Cornell University Ph.D., Rockefeller University Office: 252 PSB Phone: (831) 459-2067 Fax: (831) 459-2935 schleich@chemistry.ucsc.edu

Department of Chemistry University of California 1156 High Street Santa Cruz, CA 95064 Office Hours Class Times & Locations Labs: 290 PSB, 9-4382 Other Pursuits

RESEARCH INTERESTS: Biomedical Magnetic Resonance, In Vivo Magnetic Resonance Spectroscopy and Imaging

The research interests of Thomas Schleich focus on the development, validation, and implementation of magnetic resonance based techniques for the detection and management of human acute and chronic disease states. Our strategy follows a theme of theory, computer simulation, experimental validation, and application.

Present interests include the development of computer optimized slice selective RF pulses for use in MR experiments at high field. Pulse sequences under development, include excitation pulses with immunity to RF B₁ inhomogeneity, and concatenated selective pulses. Potential improvements to multidimensional pulse design are also being explored. Surface coils and various surface coil arrangements are used widely in both human and animal experiments at high magnetic field strengths, and knowledge of their excitation and reception fields is essential for optimal design and use. Thus, in addition to RF pulse development, computer simulation of the excitation and reception sensitivity fields for a variety of surface coil configurations, including dual surface coils (ranging from circular to square coils) and non-planar coils, driven in quadrature, are under development. Additionally, excitation and reception fields for the B₁ insensitive excitation of these coils will be simulated using selected optimized RF pulses. Methods to incorporate the effects of tissue electrical properties on the excitation and reception sensitivity fields of these coils are also considered. (These studies are in collaboration with Dr. G. B. Matson and affiliated members of the Magnetic Resonance Unit, San Francisco VA Medical Center.) Other ongoing research involves the development of neural network based schemes for the identification of spectral contributions from specific metabolites in in vivo NMR spectra.

Schleich's past research interests included proton magnetization transfer in crowded macromolecular environments, the development and refinement of the off-resonance rotating-frame spin-lattice relaxation experiment for the investigation of macromolecular rotational diffusion, and the physical biochemistry of ocular lens transparency.

SELECTED PUBLICATIONS

Y. Luo, J. Rydzewski, R. A. de Graaf, R. Gruetter, M. Garwood, and T. Schleich, "In Vivo Observation of Lactate Methyl Proton Magnetization Transfer in Rat C6 Glioma," Magn. Reson. Med. 41, 676-685, 1999. (PDF version)

K. Kuwata, H. Liu, T. Schleich, and T. L. James, "Rotational Correlation Times of Internuclear Vectors in DNA Duplex with G-A Mismatch Determined in Aqueous Solution by Complete Relaxation Matrix Analysis of Off-resonance ROESY (O-ROESY) Spectra," J. Magn. Reson. 128, 70-81, 1997. (PDF version)

S. T. Crooke, M. J. Graham, J. E. Zuckerman, D. Brooks, B. S. Conklin, L. L. Cummins, M. J. Grieg, C. J. Guinoso, D. Kornbrust, M. Manoharan, H. M. Sasmor, T. Schleich, K. L. Tivel, and R. H. Griffey, "Pharmacokinetic Properties of Several Novel Oligonucleotide Analogs in Mice," J. Pharm. Exper. Therapeutics 277, 923-937, 1996.

J. A. Willis and T. Schleich, "Oxidative-Stress Induced Protein Glutathionine Mixed-Disulfide Formation in the Ocular Lens," Biochim. Biophys. Acta 313, 20-28, 1996.

J. M. Rydzewski and T. Schleich, "Deuterium Off-Resonance Rotating Frame Spin-Lattice Relaxation of Macromolecular Bound Ligands," Biophysical J. 70, 1472-1484, 1996.

D. Brooks, P. Ladam, K. Kuwata, and T. Schleich, "Re-evaluation of the Off-Resonance Rotating-Frame nuclear Overhauser Effect (O-ROESY) Experiment," J. Magn. Reson. A, 117, 307-310, 1995.

K. Kuwata and T. Schleich, Polarization Operator Formalism Description of the Off-Resonance ROESY Experiment, J. Magn. Reson. A, 114, 219-229, 1995.

J. A. Willis and T. Schleich, "¹³C NMR Spectroscopic Studies of 2-Mercaptoethanol Stimulated Glutathione Synthesis in the Intact Ocular Lens," Biochim. Biophys. Acta 1265, 1-7, 1995.

A. Stevens, S. X. Wang, G. H. Caines, and T. Schleich, ¹³C NMR Off-Resonance Rotating Frame Spin-Lattice Relaxation Studies of Bovine Lens gamma-Crystallin Self-Association: Effect of 'Macromolecular Crowding'," Biochim. Biophys. Acta 1246, 82-90, 1995.

K. Kuwata and T. Schleich, "Off-Resonance Rotating Frame Nuclear Overhauser Effect Spectroscopy," J. Magn. Reson. Series A, 105, 43-49, 1994.